Question

Several medically important protein pharmaceuticals have been produced using animal cell culture and recombinant DNA technology. Represent the animal cell line used for the production of the following proteins and their therapeutic use in a tabular form.

(a) Erythropoietin

(b) Factor VIII

(c) Follicle Stimulating Hormone (FSH)

(d) Interleukin 2 (IL 2)

(e) Monoclonal antibodies (mAbs)

Answer 1

+------------------------------------+-----------------------+--------------------------------------+
|   Proteins                         | Animal cell line used |      Therapeutic use                 |
+------------------------------------+-----------------------+--------------------------------------+
| Erythropoietin                     | CHO cells             | Anemia                               |
+------------------------------------+-----------------------+--------------------------------------+
| Factor VIII                        | CHO cells             | Hemophilia A                         |
+------------------------------------+-----------------------+--------------------------------------+
| Follicle Stimulating Hormone (FSH) | CHO cells             | Infertility                          |
+------------------------------------+-----------------------+--------------------------------------+
| Interleukin 2 (IL 2)               | CHO cells             | Cancer therapy                       |
+------------------------------------+-----------------------+--------------------------------------+
| Monoclonal antibodies (mAbs)       | Hybridoma cells       | Cancer therapy & Autoimmune diseases |
+------------------------------------+-----------------------+--------------------------------------+